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genoCDS is a clinical reference tool for qualified healthcare professionals only. Content is derived from published guidelines and regulatory sources and may contain errors or outdated information. It is not a substitute for professional medical judgment, institutional protocols, or individual patient assessment. Always verify recommendations against current primary sources before making clinical decisions. Full disclaimer →

Colorectal Cancer · Colon Adenocarcinoma

Stage Stage IIIA

Ipsilateral mediastinal or subcarinal nodal involvement, or T3-4/N1-2/M0. Potentially resectable in select cases; often requires multidisciplinary evaluation.

3 test recommendations across 1 clinical indication

At Diagnosis

Stage IIIA (T1-T2, N1, M0) colon cancer. MSI-H/dMMR identifies Lynch syndrome candidates; BRAF V600E aids interpretation.

Required 1 test

RequiredFDA Companion Dx

Microsatellite Instability / Mismatch Repair Status (MSI/MMR)

MSI/MMR

LOINC 81695-4

Testing for microsatellite instability (MSI) and/or mismatch repair (MMR) deficiency. Methods: PCR-based MSI testing, IHC for MMR proteins (MLH1, MSH2, MSH6, PMS2), or NGS-based MSI calculation. MSI-H (high instability) / dMMR (deficient MMR) is an FDA-approved pan-tumor biomarker for pembrolizumab and dostarlimab. MSI-H/dMMR is rare in NSCLC (<1%) but justifies routine testing given pan-tumor approval. IHC for MMR proteins can be performed on most tissue specimens.

Ordering Note

Required for all Stage III CRC (NCCN Cat. 1). MSI-H informs adjuvant chemotherapy choices and Lynch syndrome assessment.

Specimen

FFPE tumor tissue (≥10% tumor)

Evidence

NCCN
NCCN CRC v4.2024
NCCN Cat. 1

Recommended 1 test

RecommendedFDA Companion Dx

BRAF V600E Mutation Analysis

BRAF V600E

LOINC 81527-9

Detection of BRAF V600E (p.Val600Glu) point mutation. Dabrafenib + trametinib (Tafinlar + Mekinist) is FDA-approved for BRAF V600E-mutant metastatic NSCLC. BRAF V600E occurs in 1.5–4% of NSCLC, more commonly in adenocarcinoma, and is enriched in current/former smokers. Non-V600E BRAF mutations occur more frequently but currently lack approved targeted therapy.

Ordering Note

Recommended alongside MSI testing. BRAF V600E in MSI-H context distinguishes sporadic from Lynch syndrome. In MSS tumors, strong negative prognostic marker.

Specimen

FFPE tumor tissue (≥10% tumor)Liquid biopsy (ctDNA)

Evidence

NCCN
NCCN CRC v4.2024
NCCN Cat. 2A

Optional 1 test

OptionalFDA Companion Dx

Comprehensive Solid Tumor NGS Panel

Comprehensive NGS

LOINC 81445-4

Next-generation sequencing panel covering ≥300 cancer-relevant genes, including all major NSCLC drivers (EGFR, ALK, ROS1, BRAF, KRAS, MET, RET, NTRK, HER2, FGFR), plus tumor mutational burden (TMB) and microsatellite instability (MSI). Examples include FoundationOne CDx, Tempus xT, Caris MI Transcriptome, and equivalent institutional panels. Preferred over single-gene sequential testing for efficiency and cost-effectiveness.

Ordering Note

Consider to identify actionable alterations (KRAS G12C, HER2, NTRK) that may become relevant at recurrence.

Specimen

FFPE tumor tissue (≥20% tumor)Liquid biopsy (ctDNA)Fresh/frozen tumor tissue

Evidence

NCCN
NCCN CRC v4.2024
NCCN Cat. 2B

Payer Coverage

Payer Coverage Summary

Coverage status as of last policy review. Prior authorization requirements and coverage criteria may change. Verify directly with each payer before ordering.

TestMedicare (CMS)UnitedHealthAnthem BCBSHumanaCignaAetna
MSI/MMRCoveredCoveredUnknownUnknownUnknownUnknown
Comprehensive NGSCoveredCoveredCoveredPrior AuthCoveredCovered

Coverage data last verified via automated policy research. Always confirm current policies with each payer.

Reference only. Testing guidance shown is derived from published clinical guidelines and regulatory sources. It does not constitute a clinical recommendation for any individual patient. Payer coverage information is a general summary and may not reflect current policy or individual benefit design. Full disclaimer

Recommendations on this page are derived from publicly available guidelines (NCCN, ASCO, ESMO, FDA, OncoKB) and are paraphrased for reference. They do not constitute medical advice. Evidence levels reflect the grading systems of each respective organization and should be interpreted in clinical context. This reference is updated periodically but may not reflect the most recent guideline revisions.

Colon Adenocarcinoma — Stage IIIA | Colorectal Cancer | genoCDS | genoCDS